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After dispensing into 96-well white plates, a baseline read of luminescent signal was serially recorded over 5 min using a Flexstation 3 instrument (Molecular Devices) at 37C before addition of the indicated concentration of ligand, after which signal was repeatedly recorded for 30 min

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observed significant SAT reduction without corresponding VAT changes [39]

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10.1007/s10541-005-0104-5 Biochemistry ( Mosc