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2018 For more Drug Warnings (Complete) data for Methylene blue (20 total), please visit the HSDB record page
Subsequently, immunostaining was visualized with diaminobenzidine and slices were counterstained with hematoxylin
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The PCR products were digested with Nco I and Bam HI and ligated into the pKM260 expression vector (EUROSCARF, Frankfurt, Germany), which resulted in the N-terminal addition of a hexahistidine tag and linker with the sequence MHHHHHHASENLYFQGAM