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For sequencing, the V4V5 region of the 16S rRNA gene was amplified from the extracted DNA using com1 (CAGCAGCCGCGGTAATAC) and com2 (CCGTCAATTCCTTTGAGTTT) primers (Lane et al., 1985), as previously described (Macey et al., 2023)

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14 Postprandial GLP-1 levels are markedly increased in patients after bariatric surgery as compared with those who have not undergone surgery and are even higher in most patients with PBH

The researchers adjusted for a variety of other factors, including serum cholesterol, lifestyle, and body mass index

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Wolffenbuttel BHR et al