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50 g/mL) was added to the cell suspension and incubated for an additional 5 min in the dark

"Methylation of nicotinamide with soluble enzyme system from rat liver (in Eng)

2.5.2 cDNA synthesis and RT-qPCR Reverse transcription was conducted with 40 ng of RNA per sample using the Reverse Transcription Master Mix Kit (Fluidigm Corporation, San Francisco, California, USA), following the manufacturers instructions

10.1016/j.cell.2008.06.028 29 CaiW

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