Maintenance of Cell Phenotype To determine maintenance of biochemical phenotype, cryopreserved primary canine hepatocytes plated as described above were incubated for 24, 48, or 72 h
Allocati N, Masulli M, Di Ilio C, Federici L (2018) Glutathione transferases: substrates, inihibitors and pro-drugs in cancer and neurodegenerative diseases
In a healthy, balanced system, an enzyme called glutathione reductase "recharges" the depleted GSSG back into the active GSH form
TMT liquid chromatography-mass-spectrometry (LC-MS) analysis Samples were analyzed by liquid chromatography-tandem mass spectrometry using an Orbitrap Fusion Tribrid Mass Spectrometer (Thermo Scientific) coupled to an UltiMate 3000 Series Rapid Separation LC system and autosampler (Thermo Scientific Dionex)
It is available through licensed medical providers for investigational and compounded use
doi: 10.1111/1750-3841.17300 5 TomookaFKajiKNishimuraNKuboTIwaiSShibamotoAet al