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Their germ-free mice maintenance protocol is as follows: mice are maintained in a vinyl isolator (CBC, Madison, WI) at the room the temperature of 22 1 C and 5565% relative humidity with a 12-h light/12-h dark cycle

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We next examined the mRNA levels of glycolytic key rate-limiting enzymes (HK2), TCA rate-limiting enzymes (IDH2 and PDH), lipid uptake and transport-related enzymes (CD36 and Slc27a1) and fatty acid -oxidation (PDK4, ACOX1 and ACOT1) (Fig

In resting cells, GHK-Cu produces modest ERK1/2 activation supporting proliferative responses, while in inflammatory conditions, the peptide suppresses p38 and JNK activation, contributing to anti-inflammatory effects