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Oil Red O staining kit (Solarbio Science and Technology Co., Beijing, China) for lipid staining in LO2 cell
After fixing in 4% paraformaldehyde for 10 min, the fluorescent probe DCFH-DA (Beyotime Biotechnology, #S0033) and JC-1 dye (Beyotime Biotechnology, #C2006) were used for evaluations of ROS and mitochondrial membrane potential as per their manufacturers instructions
Wolf et al., 2005)
[PMC free article: PMC3111292] [PubMed: 21606363] 844
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New model systems and the development of targeted therapies for the treatment of neurofibromatosis type 1-associated malignant peripheral nerve sheath tumors
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