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Preparation of DAB-stained HEK293T cells for EM LaccID-expressing cells were fixed using warm 2% glutaraldehyde in buffer (100 mM sodium cacodylate with 2 mM CaCl 2 , pH 7.4) and then quickly moved to ice

Cells were cultured in high-glucose DMEM supplemented with 10% heat-inactivated fetal bovine serum (FBS) and 1% penicillin-streptomycin, and maintained in a humidified incubator at 37 C with 5% CO

Supplementary information Rights and permissions About this article Cite this article Peters, Y., Al-Kaabi, A., Shaheen, N.J

The biochemistry of natural fasting at its limits

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