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Tissue fixation and preparation of paraffin-embedded, frozen, and vibratome-cut sections Brain hemispheres were fixed by immersion in 4% paraformaldehyde in phosphate buffer solution (Nacalai tesque, Kyoto, Japan)

W.ten BergeD.ChenH.GuX.et al (2007)

After discarding the supernatant, islets were incubated in KRBH containing 0.1% BSA, 1 mM glucose and indicated concentrations of somatostatin (SST-14) or insulin at 37 C for 1 h

The respective primary antibodies and HRP-conjugated secondary antibodies were diluted 1:2,000 in Can Get Signal Solution 1 (TOYOBO) and 1:5,000 in Can Get Signal Solution 2 (TOYOBO), respectively

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While such reactions are uncommon, they require immediate medical attention