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To evaluate spermatogenesis and male fertility in mice, Computer-Aided Sperm Motion Analysis (CASA) was conducted on epididymal sperm to determine motility parameters, including the percentage of motile and forward progressive sperm

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Blots were incubated in chemiluminescent HRP substrate (Millipore) and then imaged with iBright FL1500 Imaging System (Thermo Fisher, Fair Lawn, NJ)

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Dose Range: 100300 mcg daily with gradual titration