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Recent data have indicated that such an effect may depend on the influence on metabolic processes because in chronic unpredictable mild stress (CUMS) in mice, the decreased GLP-1 level in the hippocampus was increased with metformin treatment [46]

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As a loading control for ubiquitinated protein expression, nitrocellulose membranes were stained with 0.5% Ponceau S (Sigma-Aldrich) in 1% acetic acid

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