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After incubation, cells were stained with propidium iodide (PI, # 40710ES03, Yeasen) solution (40 g/mL in PBS) for 15 min at room temperature and the cell cycle was analyzed using Flowsight flow cytometer (Merck Millipore, Darmstadt, Germany)

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But otherwise, in the general population, I see no relevant evidence for concerns, and these are the patients I normally see, Klonoff said

Metformin is associated with higher relative abundance of mucin-degrading Diabetes Care 2017;40:54-62

Oz N, Vayndorf EM, Tsuchiya M, McLean S, Turcios-Hernandez L, Pitt JN, et al

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