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Immunohistochemistry (IHC) and immunofluorescence (IF) staining were performed on deparaffinized and rehydrated lung sections

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Male adult-born neurons mature faster compared with female adult-born neurons We then examined whether there are sex differences in maturation rate of adult-born neurons by examining the percentage of BrdU-ir cells expressing maturation stage specific neuronal markers, immature neurons (DCX) and mature neurons (NeuN) across the 3 weeks