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The V5/His6 tags were inserted in-frame at the C terminus before the stop codon of each coding sequence using the reverse primers ( RL11 -( BamHI )-rev and ICOS-L 1-268 ( BamHI )-rev)

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Abbas et al., 2023a) and exhaustion of antioxidant defenses, like downregulation of GSH and the Nrf2/heme oxygenase-1 signaling (Bu et al., 2018)