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To identify BPC157-FBXO22-dependent effectors, we treated HUVECs with BPC157 for 24 h and analyzed protein expression via Western blotting

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For visualisation of covalent PDE adducts by western blot, SDS-PAGE was run with mPAGE TM Bis-Tris precast gels (412% or 420% acrylamide) in mPAGE TM MES running buffer at 125 V constant until maximum band resolution