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Breaking it requires strict sleep hygiene: same wake time every day regardless of how much sleep occurred, no screens an hour before bed, bedroom used only for sleep

GI effects are most pronounced

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The in vitro experiments were performed using TM3 mouse Leydig cells that were cultured in DMEM/F-12 medium supplemented with 5% horse serum and 2.5% fetal bovine serum, and maintained at 37C under a 5% CO2 atmosphere

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