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However, these issues can sometimes be circumvented, for example through N -alkylation, 8 inclusion of D-amino acids or use of disulfide, ring closing metathesis (RCM) or lactam formation as cyclisation approaches

doi: 10.1016/j.cell.2020.05.048 106 KitamuraK.ShionoyaH.SuzukiS.FukaiR.UdaS.AbeC.et al

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Increased risk of contamination : Higher temperatures promote bacterial growth in reconstituted solutions