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The flow rate was maintained at 0.15 ml min 1 with the following linear gradient: 023 min from 95% buffer B to 5% buffer B, 2325 min hold at 5% buffer B, 2525.5 min transition to 95% buffer B at 0.20 ml min 1 , 25.532.5 min hold at 95% buffer B and 32.533 min at 95% buffer B with a 0.15 ml min 1 flow rate

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Compositional changes in lignocellulosic content of some agro-wastes during the production cycle of shiitake mushroom

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