Food Chem Toxicol 46:10791088 Acknowledgements The authors would like to thank Professor Zubeyde BAYSAL (Department of Chemistry, Faculty of Science, Dicle University, Diyarbakir/Turkey) for generously providing laboratory facilities and some needed reactants
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The liver tissue was homogenized with a buffer solution having 250 mM sucrose, 50 mM Tris-HCl (pH 7.4), and 1 mM EDTA and stored with 4% paraformaldehyde (PFA) or at 80C
As a group, the toxins possess quite different biological activities including inhibition of voltage-gated calcium and sodium channels, insecticidal activity, lectin-like agglutination, and inhibition of trypsin
The symptoms of a more severe deficiency include anemia, which presents as fatigue, pale skin color, shortness of breath, and lightheadedness