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Epinephrine was added to the assay mixture, containing tissue supernatant, and the change in extinction coefficient was followed at 480 nm in a spectrophotometer

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Glutathione peroxidase (GPx) is an enzyme with peroxidase activity whose main biological role is to protect the cell from oxidative damage by reducing lipid hydroperoxides to their corresponding alcohols and reducing free hydrogen peroxide to water [21]

Persistent inflammatory factors stimulate HSCs in the liver to secrete fibrotic factors that increase extracellular matrix (ECM) formation and ultimately lead to liver fibrosis (Eraky et al., 2018)