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10.1371/journal.pone.0072849 230 ZenderL.SpectorM

Chilis has had a well-publicized comeback over the last two years, and for GLP-1 users, the upgraded menu is actually good news

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10.1172/JCI122313 91 KishiS.KadoyaH.KashiharaN

To quantify mRNA, following cDNA synthesis, the quantitative RT-PCR (qRT-PCR) analysis was carried out on an Applied Rotor-Gene 3000 with the following conditions: GAPDH and target genes were amplified for 40 cycles at 94C for 30 seconds, 63C for 60 seconds, and 72C for 90 seconds