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Methylenblau 43

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The cells were washed twice with PBS and incubated with 1 mM of 2-DG for 10 min at 37 C

Measurements of intracellular ROS After treatment with IL-22 (25 ng/ml) and/or SFN (20 nM), HaCaT cells were harvested and stained with 2 M dihydroethidium (DHE, Beyotime Institute of Biotechnology) for 30 min at 37 C in the dark

Typical daily dose: 5,000 mcg (5 mg) once daily for 20 consecutive days

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