Discuss this approach with your provider
The pipette solution for whole-cell recordings was modified to include an intracellular dye (Alexa Fluor 350 hydrazide dye) and contained: 120 mM K-gluconate, 10 mM KCl, 10 mM HEPES, 5 mM EGTA, 1 mM CaCl 2 , 1 mM MgCl 2 and 2 mM MgATP, and 0.03 mM Alexa Fluor 350 hydrazide dye (pH 7.3)
While at pH value greater than 6.5, the decrease might be due increased ionization of GSH which made the non-enzymatic rate product formation much rapid to upset the enzymatic rate
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Diet, lipoproteins, and coronary heart disease