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Cells synthesize glutathione in the cytosol, rather than on ribosomes, and binding of the amino group of cysteine to the - rather than to the -carboxyl group of glutamic acid makes the bond inert towards peptidases

This approach has merit but still works indirectly and much more slowly than IV therapy

Likewise, targeted disruption of key carnitine shuttle enzymes mediating transport of acetyl-CoA to the cytosol of lymphoma cancer cells for fatty acid synthesis, was shown to cause a cytotoxic depletion of acetyl-CoA and growth inhibition in these cells 69

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[DOI] [PMC free article] [PubMed] [Google Scholar] 80.Gong Y., Wang G., Gong Y., Yan J., Chen Y., Burczynski F.J