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The HEK293 cell line expressing a functional mouse GLP-1R and the HEK293 cell line (generated in-house using HEK293 cell line purchased from ATCC) expressing a functional human glucagon receptor were cultured in DMEM supplemented with 10% fetal bovine serum and 10 g ml 1 blasticidin

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The role of GLP-1R in DM is principally to regulate glycemic control by stimulating insulin secretion, inhibiting glucagon secretion, and improving insulin signaling, thereby reducing blood glucose levels

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