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Residual cells, debris were discarded by centrifugation and CM concentrated with concentricon devices (Millipore, Temecula, CA) with a 3-kDa membrane pore cut-off, which eliminates the residual ALCAR

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10.1016/S0731-7085(03)00573-9

These approaches involve the use of stem cells and biomaterials to create a supportive environment for cellular growth and differentiation