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Experimental approachesCC50 calculation of phenformin and atpenin A5 CC 50 calculations were performed using three methods to measure cell viability: the MTT assay, the CellTiter-Glo assay (CTG) from Promega (Cat# G7570), and monitoring cell viability via live cell imaging (IncuCyte)

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Extracellular matrix and collagen research Beyond the wound-healing context, GHK-Cu is used in research on extracellular matrix biology more generally including collagen synthesis, the cross-linking reactions catalyzed by lysyl oxidase, the deposition and remodeling of matrix in various cell-culture systems, and the broader matrix metabolism that connects to tissue mechanics and tissue function