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The mixtures were thoroughly vortexed, allowed to hydrate for 1 h, then centrifuged (5600 g , 30 min, 2325 C) and the supernatant collected

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Results Characteristics of the study subjects The background characteristics of 40 subjects in this study were as follows: Male/female =27/13, abdominal or thoracic aortic aneurysm/aortic dissection/arteriosclerosis obliterans/other = 12/20/6/2, diabetic/non-diabetic = 13/26, age 68.0 13.4 years old, BMI 23.8 4.7 kg/m 2 , HbA1c 6.2 1.5%

Drinking alcohol heavily Alcohol causes a chemical irritation of the larynx that produces changes similar to those seen in smokers