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This research was supported by NIH grant RO1 CA105437 (C.M.U.) and NSF grant DMS-0616710 (M.C.R.)

Expected molecular weight and purity were confirmed on SDS-PAGE by the standard protocol and HPLC-SEC column (AdvanceBio SEC 300 A 2.7 m 4.6 150 mm, Agilent) on 1260 Infinity II (Agilent)

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Direct binding of the Corrector VX-809 to Human CFTR NBD1: Evidence of an Allosteric coupling between the Binding site and the NBD1:CL4 Interface s