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Murine cell lines were cultured in DMEM/F12 (ThermoFisher, #A4192001) while patient-derived cell lines were cultured in OptiMEM (ThermoFisher, #31985070)

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In turn, this results in an increased synaptic excitation/inhibition ratio, which may contribute to the development of ASD phenotypes (67)

Meanwhile, compared with unprocessed S

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