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After incubation, the sample was diluted to 80% ACN, 1% TFA and incubated on a shaker at RT for 10 min, to allow the phosphopeptides to re-attach to the TiO 2 beads

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But he notes that there are some key differences

hGLP-1, pGLP-1, eGLP-1 and Ex-4 (7 M) were incubated with purified human serum in 37 C incubator in the presence/absence of 100 M of DPP-4 inhibitor P32/98 (Santa Cruz Biotech), and aliquots of the reaction solutions were extracted after 0, 3, 6, 9 and 11 h incubation respectively, when TFA (0.1%) was added to terminate the reactions

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