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Effective systemic dose in pigs of 1.1 mg/kg (~75 mg in humans) showed no adverse effects, representing approximately 300-fold below toxic dose

An open trial that included 32 women with PCOS found a multi-nutrient supplement providing 400 mcg (680 mcg DFE) 5-MTHF daily for three months decreased homocysteine levels compared with no treatment

Palmos AB, Duarte RRR, Smeeth DM, Hedges EC, Nixon DF, Thuret S, et al

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The NHS advises that 0.35 mg (350 g) or less a day from supplements is unlikely to cause harm, but selenium has a narrow safe window, so more is not better