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Micro BCA Protein Assay Kit, H 2 DCFDA (H 2 -DCF, DCF), C11-BODIPY (581/591), MitoSOX Red Mitochondrial Superoxide Indicator, MitoTracker Red CMXRos, and Dulbeccos modified Eagles medium (High glucose, no glutamine, no methionine, no Cys 2 ) were purchased from Thermo Scientific (Waltham, MA, USA)

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Based on current studies, targeted agents inhibiting PAF and its receptor needed more research because of the limitation of experience with the therapeutic effects of this target

When released into the bloodstream, circulating DPP3 (cDPP3) cleaves various peptides of the RAS, including Ang II [14, 15]

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After three washes with PBS-T, the membrane was incubated with AP-conjugated anti-mouse or anti-rabbit IgG antibody for 1 h