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Consistent with the suppression of mTORC1 signaling, we observed that cystine starvation markedly activated the ISR (Fig

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Ice-cold MeOH (600 L), CHCl 3 (200 L), and H 2 O (500 L) samples were vortexed and then centrifuged (10,000 g , 10 min, 4 C) to precipitate proteins

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