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Concentration formula Concentration (mg/mL) = Total peptide (mg) / Total water volume (mL) For a 10 mg vial: 10 mg / 2 mL = 5 mg/mL 10 mg / 3 mL = 3.33 mg/mL 10 mg / 4 mL = 2.5 mg/mL 10 mg / 5 mL = 2 mg/mL Dose volume formula Volume needed (mL) = Desired dose (mg) / Concentration (mg/mL) For 0.5 mg at 3.33 mg/mL: 0.5 / 3.33 = 0.15 mL Syringe units formula Units = Volume (mL) x 100 For 0.15 mL: 0.15 x 100 = 15 units Combined formula (all-in-one) Units = (Desired dose in mg / Concentration in mg/mL) x 100 This is the only formula you actually need

CRediT authorship contribution statement Wentian Lu: Conceptualization, Data curation, Investigation, Methodology, Visualization and Writing - original draft

doi: 10.7861/clinmedicine.15-2-145 75 BrasitusTA

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Mortality of the high dose arctigenin-treated group (50 mg/kg) was 22.5%, compared to AFE and Arctii fructus water extract (AFW) groups (100 mg/kg), which were 20% and 42.5%, respectively

For general liver health, one capsule per day is typically recommended, though you should always check with your healthcare provider if youre taking medications, as milk thistle can interact with some drugs