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lactis LL-plSAM-FVpE, the SAM-FVpE protein or the FVpE protein were injected into the ileal loops respectively, and the fluorescent signals for FVpE were monitored by using rabbit anti-FVpE antibody and Alexa Fluor 647 Goat anti-rabbit IgG antibody

Upon addition of ME, the olefinic proton (H a ) of TPE-DCV with chemical shift of ~8.4 ppm disappears and a new peak (H b ) at 4.69 ppm arises, suggestive of the addition reaction of thiol on the vinyl group (Figure S6) 58

Although critical glutathione levels will lower as we age, depletion can be accelerated by stress, illness, infection, poor diet, alcohol, cigarettes, trauma, and environmental toxins

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High-performance liquid chromatography confirms peptide identity and purity percentage

Our overall goal is to provide a roadmap towards developing metabotherapies for intestinal disease