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Physiol Behav 52:185187 Aureli T, Miccheli A, Ricciolini R, Di Cocco ME, Ramacci MT, Angelucci L, Ghirardi O, Conti F (1990) Aging brain: effect of acetyl-l-carnitine treatment on rat brain energy and phospholipid metabolism
Cells were washed with PBS and fixed with 4% paraformaldehyde (PFA) for 10 minutes at 4C, permeabilised for 15 seconds in a liquid nitrogen bath, blocked in 1% BSA / PBS for 1 hour at room temperature, incubated with D4H*-mCherry (1:25 in 1% BSA / PBS) for 2 hours, washed with PBS and post-fixed in 4% PFA for 10 minutes at room temperature
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Various matrices have shown strong performance in glycolipid analysis
Tambin acta en el cerebro, donde los receptores a los que se une se concentran en regiones que regulan la recompensa, la motivacin y el estrs: los mismos circuitos que son alterados por la adiccin
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