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Three different mechanisms (gene expression, angiogenesis, cell migration) engaging three complementary biological pathways

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Low concentrations (1-2mM) of other additives such as Mg2+ or Ca2+ in Solution B can also be maintained constant in the gradient by inclusion in Solution A at 3-6 mM

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Nat Rev Cancer 15(9):540555 Ruhaak LR, Xu G, Li Q, Goonatilleke E, Lebrilla CB (2018) Mass spectrometry approaches to glycomic and glycoproteomic analyses