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C-met binding was examined at two HGF-doses (250 ng/ml and 500 ng/ml in PBS) by sandwich ELISA-like assay on recombinant c-met-Fc chimera as in Figure 1e, and significant changes in optical absorbance subtracted by a baseline level (negative control without HGF, bar 1 ) were depicted in Figure 2a (left column)

K., Hellemann, G., Green, M

Oxidative stress occurs when there is an imbalance between free radicals (unstable atoms that can damage cells) and antioxidants

Western Blot Analysis Cellular and cerebral samples were lysed in RIPA (Beyotime, China) containing inhibitor cocktail (Roche, Germany) for 20 min, following which the lysates were centrifuged (12,000 g) at 4C for 15 min

Unlike a pure cell culture (where individual cells grow in a dish), ex vivo organ culture preserves the complete tissue structure in this case, whole hair follicles with all their cell types intact

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