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After three washes, the AP substrate consisting of 5-bromo-3-chloro-indolyl phosphate (Roche Diagnostics, #10760978103) and 4-nitro blue tetrazolium chloride (Roche Diagnostics, #11383213001) in the AP buffer was added for detection

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Additionally, dietary potassium and sodium should be controlled according to the comorbidities, medicine use, blood pressure and test results