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Off / washout (after the block): A typical research framing runs a defined block (commonly 812 weeks) followed by an off period before any subsequent block, rather than indefinite continuous use because long-term human data simply do not exist

Usable blastocysts were those with a score of 3BB or above according to the Gardner grading system ( Statistical analysis Statistical analyses were performed using the GraphPad Prism 6 software (La Jolla, CA, United States)

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