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The bacteria were then pelleted, resuspended in 50 mM Tris-HCl pH 7.5, 20 mM imidazole, 300 mM NaCl (lysis buffer) and frozen in liquid nitrogen
High concentrations of 4HNE have been associated with different processes such as apoptosis, cell differentiation, altered gene expression and with several diseases correlated to redox imbalance such as neurodegenerative and cardiovascular diseases, metabolic syndrome, and cancer 15
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