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TaRgeting Of Deregulated Wnt/-catenin signaling by PRI-724 and LGK974 inhibitors in germ cell tumor cell lines

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Real-time quantitative polymerase chain reaction (RT-qPCR) The samples were added into Trizol for cracking, then chloroform was added for centrifugation to obtain the upper liquid phase, and isopropyl alcohol was added for centrifugation to obtain the precipitate

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These include elevated levels of c-reactive protein (CRP), IL-6, and increased monocyte counts, suggesting that circadian disruption through NSW may promote a pro-inflammatory and pro-tumorigenic immune environment 138

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