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Samples contained 0.2 M protein in reaction buffer (100 mM K 2 HPO 4 /KH 2 PO 4 pH 7.4, 1 mM EDTA) and 10 mM total dithiothreitol (DTT) for full reduction or 10 mM total H 2 O 2 for full oxidation of the sensor, respectively

Here, we summarize recent advances in our understanding of the NAD + homeostasis in response to growth conditions or environmental stimuli, highlighting the actions of NAD + in coordinating metabolic reprogramming and maintaining cellular physiologic biology, which enables the plastic cells to adapt to environmental changes

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