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Sorting vector construction and gene cloning The DNA sequences encoding the GST, the N-terminal signal peptides, and the C-terminal membrane-placing module of the six GPI/TMDs (Supplementary Table S1) were synthesized and supplied in pUC57a by GENEWIZ

Techniques such as gene expression analysis, telomere length measurement, and immune response assays are commonly used

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In cell studies, the complex stimulates fibroblasts to produce types I, III and IV collagen, elastin and glycosaminoglycans, while down-regulating matrix metalloproteinases that break down the skins scaffold.(pubmed.ncbi.nlm.nih.gov) These actions form the biochemical basis for its cosmetic before and after effects

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Methods At the end of the eight weeks (24 h from the last dose), all rats were euthanized under full anesthesia by intraperitoneal injection of sodium thiopental (25 mg/kg), blood samples were collected from the retro-orbital plexus of animals using micro-capillary glass tubes (Abdelrahman et al