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Further, using exendin-4 labelled with Cy3, we observed binding to beta cells in the islets of C57BL/6 mice(23) (Fig

Briefly, 0.1 ml of supernatants (2.5 mg protein/ml) mixed with 0.1 ml of 15 mM DTT in 100 mM Tris/HCl (pH 9.0), was placed in a 15 ml centrifugation tube, then sealed and incubated at 37 C for 50 min

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JCI Insight 3(22):123538 Mendell JR, Sahenk Z, Malik V, Gomez AM, Flanigan KM, Lowes LP, Alfano LN, Berry K, Meadows E, Lewis S (2015) A phase 1/2a follistatin gene therapy trial for Becker muscular dystrophy