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Akt may foster tumorigenesis by multiple means [168, 169], for example, by stabilizing cellular avian myeloblastosis virus oncogene (c-Myc) and cyclin D1 or by inducing degradation of the cyclin-dependent kinase (Cdk) inhibitor, p27 kinase inhibitor protein (p27Kip1)

Cells (1.72106) were centrifuged at 2,000 g for 1 min, and the supernatant was discarded

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