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The reaction mixture (1.0 ml) contained 500 l of 0.2 M potassium phosphate buffer (pH 7.0) containing 2 mM EDTA, 50 l of 2 mM NADPH (prepared in 10 mM Tris HCl pH 7.0), 50 l of 20 mM GSSG, 350 l distilled water

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When sodium, potassium, and chloride levels get out of whack, your nerves can misfire and create that intense throbbing pain