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Sucrose preference test For clarity and adherence to the experimental timeline, the sucrose preference test was performed after these behavioral tests to assess the impact of PCPA-induced insomnia on sucrose preference in the animals

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5.1.4 Production of neurotransmitters The gut microbiota functions as an endocrine organ by producing various hormones and neurotransmitters that impact intestinal endocrine activity with the potential to regulate kidney function