If there is presence of enzymatic activity and/or spontaneous oxidation in storage, we would expect to observe lower levels of total GSH and free GSH, and elevated levels of GSSG, in the EDTA-treated sample stored at 4 o C compared to the non-deproteinized EDTA-treated plasma stored at 80 o C
From this solution, 1 mL is mixed with 0.5 mL of transformation solution (4.5 mM KCN and 0.45 mM K 3 [Fe(CN) 6 ] adjusted with 0.25 M potassium dihydrogen phosphate to pH 7.0)
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Mild water retention: Occasional during the first weeks, typically resolving as the cycle progresses
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